Journal: Cell systems

Article Title: Gut-Liver physiomimetics reveal paradoxical modulation of IBD-related inflammation by short-chain fatty acids

doi: 10.1016/j.cels.2020.02.008

Figure Lengend Snippet: List of top 10 differential gene expression pathway enrichments, ranked based on combined z and p score, in UC gut MPS as compared to healthy control MPS based on GEO disease related pathway enrichments (A), GO Biological process terms (B) or KEGG (C). (A-C) Data represent averages of 3 replicates. (D) Representative confocal images of gut MPS from the healthy gut MPS (left) and UC gut MPS (right). Top: epithelial monolayers seeded on the apical side of transwell membranes. Bottom: Co-cultured macrophages and dendritic cells seeded on the basal side of transwell membranes (E) Representative TEER values of UC and healthy gut MPS at day 0 of gut MPS development and at day 2. ****FDR<0.0001 (F) Representative PCA of multiplexed cytokines/chemokines released by each donor’s epithelium alone, their corresponding MPS, and MPS interacting with Treg/Th17 cells. (G) Collapsed heatmaps based on means representative of 3–5 replicates indicating Z scores of cytokines/chemokines expressed under each condition as described under (F). (H) Importance matrix derived from random forest analysis of cytokines/chemokines released by gut MPSs in co-culture with Treg/Th17s. For actual concentration values of individual cytokines/chemokines measured consult Figure S1.

Article Snippet: Organoids of both donors were mechanically dissociated into single cells, resuspended in EM without nicotinamide and seeded onto type I collagen/Matrigel coated 12-well 0.4 µm pore polyester Transwell inserts (Corning, 3493) at a density of 3 x 10 5 cells/Transwell.

Techniques: Expressing, Cell Culture, Derivative Assay, Co-Culture Assay, Concentration Assay